Simulation of oral absorption from non-bioequivalent dosage forms of the salt of raltegravir, a poorly soluble acidic drug, using a physiologically based biopharmaceutical modeling (PBBM) approach
Non-bioequivalent plasma focus profiles amongst completely different dosage types of the salt of raltegravir, a poorly soluble acidic drug, have been investigated utilizing biorelevant in vitro testing mixed with the business in silico software program, Simcyp®. A suspension and a pill dosage types of raltegravir potassium have been chosen because the check formulations. Whereas dissolution from the suspension was speedy, dissolution from the tablets was sluggish and delayed by pre-exposure to an acidic surroundings.
Moreover, it was assumed that gastric emptying of the tablets can be decided by the gastric motility sample as a result of the formulation is giant and didn’t disintegrate below biorelevant dissolution check circumstances. As a way to simulate in vivo dissolution and plasma focus profiles of the tablets, in vitro and in silico trials have been carried out contemplating the gastric emptying time as a key physiological issue. The impact of pre-exposure to acid within the abdomen on dissolution habits within the gut was estimated by two-stage in vitro dissolution testing. Primarily based on these outcomes, theoretical in vivo dissolution profiles have been generated for various gastric emptying instances. Plasma focus profiles for every gastric emptying time have been simulated by inputting the theoretical in vivo dissolution profiles into the in silico mannequin constructed with the medical information of the suspension.
Imply plasma focus profiles of the goal topic group have been calculated, taking the distribution of particular person gastric emptying instances into consideration. Though the pill was anticipated to have advanced in vivo dissolution habits, plasma focus profiles have been efficiently simulated by two-stage in vitro dissolution testing and the commercially accessible in silico software program when gastric emptying was taken into consideration as the important thing physiological issue. The in vitro and in silico methodology offered on this report can be a sensible method to simulate oral absorption from numerous formulations of poorly soluble weak acids and their salts.
Mannequin-Knowledgeable Drug Discovery and Improvement in Pulmonary Supply: Biopharmaceutical Pharmacometric Modeling for Formulation Analysis of Pulmonary Suspensions
For respiratory circumstances, focused drug supply to the lungs might produce larger native concentrations with decreased threat of antagonistic occasions in comparison with systemic administration. Regardless of the rising curiosity in pulmonary supply, the pharmacokinetics (PK) of medication following pulmonary administration stays to be elucidated. On this context, the appliance of modeling and simulation methodologies to characterize PK properties of compounds following pulmonary administration stays a shortage. Pseudomonas aeruginosa (PA) lung infections are immune to lots of the present antibiotic therapies. Focused remedies for pulmonary supply may very well be notably useful for these native circumstances.
On this research, we report the appliance of biopharmaceutical pharmacometrics (BPMX) for the evaluation of PK information from three investigational antimicrobial brokers following pulmonary administration of a suspension formulation. The noticed drug concentration-time profiles in lungs and plasma of the compound collection have been mixed for simultaneous evaluation and modeling. The developed mannequin describes the PK information, taking into consideration formulation properties, and gives a mechanism to foretell dissolved drug concentrations within the lungs accessible for exercise.
The mannequin was then used to judge formulation results and the impression of variability on complete and dissolved drug concentrations in lungs and plasma. The predictions recommend that these therapies for lung supply ought to ideally be delivered in a sustained launch formulation with excessive solubility for optimum native publicity in lungs for efficacy, with speedy systemic clearance in plasma for decreased threat of undesirable systemic antagonistic results. This work reveals the potential advantages of BPMX and the function it may possibly play to help drug discovery and improvement in pulmonary supply.
Simulation of oral absorption from non-bioequivalent dosage forms of the salt of raltegravir, a poorly soluble acidic drug, using a physiologically based biopharmaceutical modeling (PBBM) approach
Improvement and validation of a selective marker-based quantification of polysorbate 20 in biopharmaceutical formulations utilizing UPLC QDa detection
Polysorbates are extensively used as non-ionic surfactant in biopharmaceutical formulations. Not too long ago, the degradation of polysorbate moved into the main focus of consideration, as a result of in a number of revealed research it was described, that stability points in polysorbate containing formulations have been noticed resulting in the formation and look of sub-visible and visual particles. For that reason, monitoring of polysorbate and its degradation merchandise is of significance all through the event of parenterals. The intention of the research was to develop a way for the selective marker-based quantification of satisfactory polysorbate 20 parts of curiosity with out the necessity to apply derivatization or advanced detection strategies. A single quadrupole mass (QDa) detector was used coupled to an ultra-high efficiency liquid chromatography (UPLC) system.
Should the Bovine Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Serum Amyloid A (SAA) in samples from serum, plasma or other biological fluids.
Should the Bovine Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Serum Amyloid A (SAA) in samples from serum, plasma or other biological fluids.
Should the Human Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Human Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Human Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Mouse Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Mouse Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Rabbit Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Should the Rabbit Serum Amyloid A (SAA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse serum Amyloid A (SAA).
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse serum Amyloid A (SAA).
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse serum Amyloid A (SAA).
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mouse serum Amyloid A (SAA).
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mouse Serum Amyloid A (SAA) in samples from n/a with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat serum Amyloid A (SAA).
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat serum Amyloid A (SAA).
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat serum Amyloid A (SAA).
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rat serum Amyloid A (SAA).
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rat Serum Amyloid A (SAA) in samples from n/a with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rabbit Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Rabbit serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Rabbit Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: Quantitativesandwich ELISA kit for measuring Human Serum amyloid A, SAA in samples from serum, urine. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human Serum amyloid A, SAA in samples from serum, urine. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Rat Serum amyloid A, SAA in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Serum amyloid A, SAA in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Bovine Serum amyloid A, SAA in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Bovine Serum amyloid A, SAA in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Sheep Serum Amyloid A, SAA in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Sheep Serum Amyloid A, SAA in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Horse Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Horse Serum Amyloid A(SAA) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativesandwich ELISA kit for measuring Dog serum amyloid A (SAA) in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Dog serum amyloid A(SAA) in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Goat Serum amyloid A (SAA) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Goat Serum amyloid A(SAA) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Quantitativecompetitive ELISA kit for measuring Pig serum amyloid A, SAA in samples from serum, plasma. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativecompetitive ELISA kit for measuring Pig serum amyloid A, SAA in samples from serum, plasma. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Cattle Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle serum Amyloid A (SAA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Cattle Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle serum Amyloid A (SAA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Cattle Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle serum Amyloid A (SAA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Cattle Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Cattle serum Amyloid A (SAA) in serum, plasma and other biological fluids.
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Cattle Serum Amyloid A (SAA) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Horse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Horse serum Amyloid A (SAA) in serum, plasma and other biological fluids.
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Horse Serum Amyloid A (SAA) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of for Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of for Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of for Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of for Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Serum Amyloid A (SAA) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Serum Amyloid A elisa. Alternative names of the recognized antigen: SA-A
SAA1
PIG4
TP53I4
Amyloid protein A
Amyloid fibril protein AA
Serum amyloid A-1 protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Serum Amyloid A (SAA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mouse Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse serum Amyloid A (SAA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Methodology improvement was primarily based on a reversed phase-high efficiency liquid chromatography assay coupled to a charged aerosol detector (RP-HPLC CAD). As an alternative of a charged aerosol detector (CAD) a QDa detector was used as a way to considerably enhance the selectivity. The main focus of this research is the event of the QDa primarily based methodology for the evaluation of polysorbate 20. Modifications of the cellular section and the kind of chromatography column allowed the separation of a number of parts of polysorbate 20 from polar non-esterified to apolar larger order species. As well as, a mess of parts may very well be quantified by their particular person m/z values. The height project recognized 676 compounds which originated from polysorbate 20. A few of these have been chosen and outlined as marker parts.
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